Journal: Experimental and therapeutic medicine

Article Title: Inhibition of lipid droplet accumulation by Solanum nigrum by suppressing adipogenesis and inducing lipolysis, thermogenesis and autophagy in 3T3‑L1 cells.

doi: 10.3892/etm.2023.12032

Figure Lengend Snippet: Figure 4. Effect of SNAP on thermogenesis and autophagy in 3T3‑L1 cells. (A) Experimental design. (B) Western blot analysis in 3T3‑L1 cells treated with SNAP from D0 to D8. (C) Western blot analysis of LC3‑I and ‑II in 3T3‑L1 cells treated with SNAP from D0 to D8. *P<0.05 vs. CON group. CON, control group without the sample treatment; DMI, mixture of 0.05 mM IBMX, 1 µM dexamethasone and 10 µg/ml insulin; SNAP, Solanum nigrum aerial part extracts; p, phosphorylated; AMPK, AMP‑activated protein kinase; PGC‑1α, peroxisome proliferator‑activated receptor‑γ coactivator 1α; PRDM16, PR domain‑containing 16; UCP‑1, uncoupling protein 1; LC3, microtubule‑associated protein 1A/1B‑light chain 3.

Article Snippet: The antibodies against PPARγ (#2430), CEBPα (#8178), FABP4 (#2120), adiponectin (#2789), ATGL (#2138), HSL (#4107), Perilipin‐1 (#9349), p‐AMPK (#2535), AMPK (#5831), UCP‐1 (#14670), LC3 (#2775), β‐actin (#5125), anti‐rabbit IgG, HRP‐linked antibody (#7074) and anti‐mouse IgG, HRP‐linked antibody (#7076) were purchased from Cell Signaling (Bervely, MA, USA).

Techniques: Western Blot, Control

Journal: Journal of dairy science

Article Title: Unveiling the Regulatory Role of SIRT1 in Oxidative Stress Response of bovine mammary cells.

doi: 10.3168/jds.2024-24936

Figure Lengend Snippet: Figure 3. Short-term and long-term effects of H2O2 stimulation on antioxidant-related proteins in MAC-T cells. (A-G) Western blot analysis of protein expression levels for SIRT1, p-AMPK, PGC-1α, Nrf2, NQO1, HO-1, and p-NF-κB p65 (n = 3). Data are shown as mean ± SEM.

Article Snippet: The antibodies used in this analysis included SIRT1 (1:2,000 dilution, 13161–1-AP, proteintech); AMPK (1:2,000 dilution, 5832, cell signaling technology); p-AMPK (1:1,000 dilution, 2537, cell signaling technology); PGC-1α (1:2,000 dilution, 66369–1-Ig, proteintech); NQO1 (1:2,000 dilution, 11451–1-AP, proteintech); NRF2 (1:1,000 dilution, ab137550, abcam); HO-1 (1:2,000 dilution, ab223349, abcam); NF-κB p65 (1:2,000 dilution, 2537, cell signaling technology); p-NF-κB p65 (1:1,000 dilution, 3033, cell signaling technology); β-actin (1:2,000 dilution, ab8226, abcam); β-casein (1:1000 dilution, ab236290,abcam).

Techniques: Western Blot, Expressing